Method for isolation and identification of potato virus M, S, X, Y

Development of RT-PCR based method for detection of potato virus Y in tobacco and potato

A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies tha...

Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187

Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

IMPACT OF POTATO VIRUS Y ON LONG-TERM STORAGE OF POTATO. Principal Investigator(s)

In recent years, Potato Virus Y has reemerged as a serious disease problem in many potato production areas in the northern United States and eastern Canada. Three recently introduced cultivars, ‘Russet Norkotah’, ‘Shepody’, and ‘Silverton’ are widely grown and express mild or no symptoms when infected with PVY. The lack of symptoms often associated with these cultivars and novel, recombinant st...

Identification of silencing suppressors of potato virus M

Gene silencing is an important regulatory and defense mechanism in plants. Plant viruses evolved some proteins that suppress cellular RNA interference mechanism. In this work identification of silencing suppressors of potato virus M (PVM) is reported. The cDNA sequences of different PVM open reading frames were cloned into the Gateway-compatible binary T-DNA destination vector pMDC32, harboring...

Isolation and Identification of Infectious Bronchitis Virus from Commercial Chickens